CUSTOM ITEM: Guard Column cartridge, Bidentate C8, replacement, 2.0 mm ID x 10 mm long cartridge packed with 5 um, 300 A phase. Cogent TYPE-C Brand. 1 EA.
Additional Info:
The 40008‑PG3‑3 Cogent Bidentate C8 Guard Column Cartridge is a single replacement cartridge designed to protect analytical HPLC columns in wide‑pore (300 Å) separations, particularly for biomolecules and complex sample matrices.
Each cartridge is:
2.0 mm ID × 10 mm length
Packed with 5 µm, 300 Å TYPE‑C™ silica hydride Bidentate C8 phase
Constructed in stainless steel hardware with SS frits
Each cartridge is supplied individually in a protective Delrin™ case, which prevents contamination, drying, and physical damage prior to use.
⚠️ Requires guard column holder (sold separately)
Designed for 2.1 mm Analytical Columns
This cartridge is specifically optimized for:
2.1 mm ID analytical HPLC columns
LC‑MS and low‑flow analytical systems
Wide‑pore / biomolecule separations
Proper phase and dimension matching ensures:
Minimal band broadening
Maintained chromatographic efficiency
Consistent retention and reproducibility
Why Use a Single Guard Cartridge
A single‑cartridge format is ideal when:
Testing different guard phases across multiple methods
Using mixed pore sizes (100 Å vs 300 Å)
Pairing guard phases with different analytical columns
Replacing cartridges individually rather than purchasing packs
This provides flexibility for method development and specialized workflows.
Why Use 300 Å Guard Cartridges
The 300 Å pore size is specifically designed for:
Peptides and proteins
Large molecular weight analytes
Complex biological or matrix‑rich samples
Compared to standard 100 Å phases:
Improves accessibility for larger molecules
Reduces steric exclusion effects
Enhances recovery and reproducibility for biomolecules
Why Use Guard Column Protection
Analytical columns are exposed to:
Particulates and contaminants
Strongly retained analytes
Matrix buildup from complex samples
Irreversible adsorption
The 40008‑PG3‑3 cartridge provides a sacrificial protection layer, helping to:
Extend analytical column lifetime
Maintain consistent chromatographic performance
Reduce downtime and replacement costs
Improve reproducibility
Cartridges can be replaced quickly without disturbing the analytical column.
Bidentate C8 Guard Phase Advantages
Using the same stationary phase as your analytical column ensures:
Matched selectivity and retention behavior
No unexpected chromatographic changes
Seamless analyte transfer
Compared to C18 phases:
Reduces excessive hydrophobic retention
Improves recovery of amine‑containing analytes
Provides alternative selectivity for complex samples
Cogent TYPE‑C™ Technology Benefits
Silica Hydride Surface
Reduced silanol interactions
Improved peak shape for basic and polar compounds
Lower secondary interactions
Fast Equilibration
Reduced method cycle times
Increased analytical throughput
Durability
Resistant to hydrolysis
Long cartridge and column life
Stable under demanding analytical conditions
Typical Applications
LC‑MS biomolecule analysis
Peptides and protein separations
Pharmaceutical and bioanalytical workflows
Complex biological matrices
Analytical methods requiring wide‑pore stationary phases
Made‑to‑Order & Pricing Information
This is a custom, made‑to‑order item.
Orders cannot be canceled once placed
Custom items are non‑returnable